Fig. 5.

Overexpression of Patronin increases the MI₂₄ of flies injured at 0–7 day old. a) A representative Western blot and (b) quantitation of Western blots for Lis-1 and α-tubulin protein expression in head extracts of 0–7 day old male flies of the indicated genotypes (n = 4). e) A representative Western blot and (f) quantitation of Western blots for Patronin and α-tubulin protein expression in head extracts of 0–7 day old male flies of the indicated genotypes (n = 4). In panels A and E, protein size markers are indicated on the left in kDa. (c, d, g, and h) The Mi₂₄ of mixed-sex flies of the indicated genotypes injured using the standard TBI protocol at the indicated age (n = 12). Symbols indicate the following: box, second and third quartiles of data; +, mean; horizonal bar, median; and whiskers, minimum and maximum data points.