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GATA4-engrailed blocks MEK1-ERK1/2-induced sarcomeric organization and hypertrophy. Representative images of immunostained cardiomyocyte cultures infected with each of the indicated adenoviral constructs are shown. The left panels show anti-α-actinin antibody (orange) reactivity to demonstrate cardiomyocyte sarcomeres and gross morphology. The right panels show the same cells double immunostained for ANF (green), which appears in a perinuclear pattern.
