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. 2022 Dec 27;37(3):695–698. doi: 10.1038/s41375-022-01805-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A A representation of the structure of IMM0306, a fusion protein of CD20 mAb with the CD47 binding domain of SIRPα (V2) extracellular segment domain 1 (D1) on both heavy chains. It simultaneously binds to CD47 and CD20 of B cells. B IMM0306 binding activity on Jurkat cells: Cultured Jurkat cells were stained with IMM0306, SIRPα-Fc and hIgG1-Fc, followed by staining with a secondary antibody anti-human IgG (Fc)–FITC. Then the cells were analyzed by flow cytometry to measure the binding ability on Jurkat cells. The data was further analyzed with GraphPad Prism 8.0 software. The results showed that IMM0306 has strong binding activity on Jurkat cells. C IMM0306 binding activity on Raji-CD47KO cells: The Raji-CD47KO cell line, modified from the Raji cell line with human CD47 knockout, was established in-house with the standard gene knockout procedures. Cultured Raji-CD47KO cells were stained with IMM0306, rituximab, and hIgG1-Fc, followed by staining with the secondary antibody anti-human IgG (Fc)–FITC. Then the cells were analyzed by flow cytometry to measure the binding ability of Raji-CD47KO cells. The data was further analyzed with GraphPad Prism 8.0 software. The results showed that IMM0306 has strong binding activity on Raji-CD47KO cells.