Extended Data Fig. 5. SPP1 deficiency prevents synapse loss upon oAβ challenge.

(a) Scheme illustrating tail vein (TV) injection of 100ng S26C oAβ versus PBS in WT versus Spp1^KO/KO mice, 72 h before tissue collection and analysis. (b) Representative super-resolution images of Homer1 and Bassoon puncta colocalization in 3 mo WT and Spp1^KO/KO SR injected with oAβ versus PBS control, at 72 h post-TV injection. Scale bar represents 5 µm. (c) Quantification of Homer1/Bassoon colocalization density, represented as S26C oAβ-injected mice normalized to PBS injected mice. 1 datapoint represents the average of 1 animal (3–5 ROIs per animal, 40 cells per ROI) with a total of n = 4 mice per genotype. Data are from one experiment. P Values from two-way ANOVA, Bonferroni’s multiple comparison test. Data are shown as Mean ± SEM.

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