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. 2023 Jan 23;26(3):495–505. doi: 10.1038/s41593-022-01245-9

Fig. 1. Knock-in mouse lines enable pyramidal neuron subtype-specific recordings of cortex-wide neural activity.

Fig. 1

a, GCaMP6s expression in Fezf2-2A-CreER;Ai162 mice. Cortical labeling is largely confined to layer 5b. Axonal projections were found in multiple subcortical regions such as the striatum and the corticospinal tract. Scale bars, 100 µm. b, GCaMP6s expression in PlexinD1-2A-CreER;Ai162 mice is widespread throughout the cortex and restricted to superficial layers 2/3 and layer 5a. Axonal projections were found in striatum and corpus callosum but absent in the corticospinal tract. c, Visual sign maps from retinotopic mapping experiments. IT and PT populations showed clear retinotopic responses in primary and secondary visual areas where boundaries largely resembled known areas from nonspecific PyNs (EMX). d, Total variance maps from same mice as in c, showing most modulated cortical regions in each PyN type.