Figure 2.

HnRNPs are required for GSC self-renewal. (A) The FLP/FRT technique was used to generate genetic mosaics. Mitotic recombination is mediated by heat-shock-induced expression of flippase (hsFLP). Homozygous mutant (mut) cells are identified by the absence of a GFP marker, which is linked to the wild-type allele. (B-E) Mock mosaic control (B), sqdix50 (C), hephe2 (D), or Hrb27CrF680 (E) mutant mosaic germaria labeled with anti-GFP (green; wild-type cells) and anti-Hts+anti-LamC (red; fusomes, follicle cell membranes, and cap cell nuclear envelopes). GSCs are outlined in white (wild-type = solid line; mutant = dashed line); asterisks indicate cap cells. Arrows indicate GFP-negative cysts. Scale bar = 5 μm. (F-G) Sibling control (F) or Df(3L)Hrb87F mutant germaria labeled with anti-Vasa (green; germ cells), anti-Hts (red), and anti-LamC (red). GSCs are outlined. (H) *p < 0.05, **p < 0.001; Chi-squares test (F, H) or Student’s two-tailed T-test (G)