FIG. 2.

Expression pattern and subcellular localization of mouse Neuralized. (A) Poly(A) RNA Northern blot analysis of mouse tissues with a mouse Neuralized cDNA probe detects a transcript of approximately 4 kbp in adult brain and a slightly smaller transcript in skeletal muscle. (B) In situ hybridization on day 10.5 p.c. mouse embryos using a mouse Neuralized probe shows expression of mouse Neuralized in the somites. (C) Dorsal view of the same embryo as shown in panel B at higher magnification. (D) Littermates were hybridized to a mouse myogenin probe as a positive control. Mouse Neuralized is expressed in the dermomyotomes of the somites in a pattern highly similar to myogenin. (E) Subcellular localization of a human Neuralized-GFP fusion protein in differentiating C2C12 cells. C2C12 myoblasts were transfected with a CMV-Neuralized-GFP construct and induced to differentiate by serum starvation. An equivalent subcellular localization was observed in a variety of different human and murine cell lines.