FIG. 8.

Analysis of mammary glands during lactation in Neuralized null females and wild-type controls. Mammary glands were harvested on the first postpartum day (L1) and analyzed by standard histological hematoxylin and eosin staining. (A) Expression of Neuralized in mammary glands during pregnancy (preg.), lactation (lact.), and regression (regres.). (B) Wild-type control mammary gland on day L1. The mammary fat pad is filled with alveoli, and lipid droplets are easily detected in the ducts. (C) Mammary gland from a Neuralized null female on L1. The mammary gland is significantly underdeveloped, with few alveoli in the fat pad. (D) Mammary gland from a Neuralized null female on L1. Alveoli appear to be more developed than in panel C; however, lipid vacuoles are not detectable in the ducts.