Figure 3.

A. Western blot analysis of frozen striatum samples assessing protein levels of B. NF-κB, C. ATF4, D. eIF2α, E. CHOP, and H. 4-HNE. While PQ significantly increased CHOP protein expression (p=0.019), which was reduced by TPPU treatment (p=0.008), increases in other proteins due to PQ treatment were not statistically significant. Results are expressed as mean ± SEM; data represent 6 biological replicates. Western Blot results were normalized to β-actin. Abbreviations: V = vehicle, PQ = paraquat, PQT = paraquat and TPPU, T = TPPU only control. Results are expressed as mean ± SEM. *P < 0.05 and **P<0.01, as determined by one-way ANOVA or Kruskal-Wallis test followed by Dunnett’s post-hoc test.