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. Author manuscript; available in PMC: 2024 Feb 27.
Published in final edited form as: Curr Biol. 2023 Jan 27;33(4):675–687.e5. doi: 10.1016/j.cub.2023.01.006

Figure 2. Pdf mRNA is sensitive to seasonal cues.

Figure 2.

(A) Pdf mRNA (magenta) was detected using fluorescence in situ hybridization (FISH) in whole brains of flies entrained for 7 days to simulated-summer (LP at 25°C) or simulated-winter (SP at 10°C). CD8::GFP was expressed using the Pdf-gal4 driver to detect the PDF-producing LNvs. (B) Representative images of LNvs of flies entrained to LP 25°C (top panel) or SP 10°C (bottom panel). (C) Quantification of the normalized intensity of the Pdf signal at ZT16 is compared between conditions. Unpaired t-test, ** p <0.01. Number of brains imaged were LP25 n = 8, SP10 n = 7 brains. Scale bar in A is 25 μm and 5 μm in B. See also Figure S1 and Table S1.