Figure 3. Locomotor adaptation to photoperiod and temperature in Pdf-null mutants.

Locomotor activity was monitored in Pdf-null mutants (yw ; Pdf⁰¹) and in flies expressing a wild-type copy of Pdf (genomic rescue, yw ; Pdf{WT} ; Pdf⁰¹) subjected to (A-B) 12:12 LD at 25°C, (C-D) 12:12 LD at 10°C, or (E-F) 8:16 LD at 25°C. (G) Variance in locomotor activity under these environmental conditions as tSNE of the normalized locomotor activity for Rescue (Resc) and the mutant (Pdf⁰¹). Each dot represents an individual fly. (H) Hierarchical clustering of the normalized locomotor activity. Arbitrary cut of the tree at 0.8 (y-axis) generates two distinct groups denotated as black and red labels. White boxes represent lights-on while black boxes represent lights-off in panels A-F. Data were analyzed using a two-way ANOVA with Bonferroni’s multiple comparison test. Number of flies used were yw ; Pdf{WT} ; Pdf⁰¹ 12:12 25°C n = 56, yw ; Pdf{WT} ; Pdf⁰¹ 8:16 25°C n = 30, yw ; Pdf{WT} ; Pdf⁰¹ 12:12 10°C n = 30, yw ; ; Pdf⁰¹ 12:12 25°C n = 55, yw ; ; Pdf⁰¹ 8:16 25°C n = 31, yw ; ; Pdf⁰¹ 12:12 10°C n = 32.