Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-PDF (clone C7-C) | Developmental Studies Hybridoma Bank | RRID: AB_760350 and AB_2315084 |
| Mouse monoclonal anti-EYA (clone 10H6) | Developmental Studies Hybridoma Bank | RRID: AB_528232 |
| Mouse monoclonal anti-BRP (nc82) | Developmental Studies Hybridoma Bank | RRID: AB_2314866 |
| Chicken Polyclonal anti-GFP | Invitrogen | Cat#A10262; RRID: AB_2534023 |
| Sheep anti-mouse IgG-HRP | GE Healthcare | Cat#NA931; RRID: AB_772210 |
| Mouse monoclonal anti-V5 | Thermo Fisher Scientific | Car#R960-25; RRID: AB_2556564 |
| Mouse monoclonal anti-FLAG M2 | Sigma-Aldrich | Cat# F3165; RRID: AB_259529 |
| Goat Anti-mouse IgG Alexa647 | Cell Signaling | Cat#4410; RRID: AB_1904023 |
| Goat Anti-chicken IgG Alexa488 | Thermo Fisher Scientific | Cat#A-11039; RRID: AB_2534096 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Schneider’s Drosophila Medium | Thermo Fisher Scientific | Cat#21720-024 |
| Fetal Bovine Serum | VWR | Cat# 97068-085 |
| Penicillin/streptomycin | Thermo Fisher Scientific | Cat#15-140-148 |
| Trypsin/EDTA | Thermo Fisher Scientific | Cat#25300-062 |
| TRI regent | Sigma-Aldrich | Cat#T9424 |
| Aprotinin | Sigma-Aldrich | Cat#A1153 |
| Leupeptin | Sigma-Aldrich | Cat#L2884 |
| Pepstatin A | Sigma-Aldrich | Cat#P5318 |
| SIGMAFAST™ Protease Inhibitor Cocktail, EDTA-FREE | Sigma-Aldrich | Cat#S8830 |
| ANTI-FLAG M2 Affinity Gel | Sigma-Aldrich | Cat#A2220 |
| PhosSTOP | Sigma-Aldrich | Cat#4906845001 |
| Normal Goat Serum | Jackson Immunoresearch | Cat#005-000-121 |
| λ-Phosphatase | NEB | Cat#P0753S |
| SsoAdvanced SYBR green supermix | Bio-Rad | Cat#1725270 |
| PBS 10X Rnase free | Invitrogen | Cat#AM9624 |
| Nuclease-free water | Invitrogen | Cat#AM9932 |
| Acetic Acid | Fisher | Cat#A38S-500 |
| Sodium borohydride (99%) | Fisher | Cat#AC448481000 |
| SSC (20X) | Fisher | Cat#AM9763 |
| Hi-Di Formamide | Fisher | Cat#4311320 |
| Triton X-100 nuclease-free | Fisher | Cat#AC327371000 |
| Formamide (deionized) | Invitrogen | Cat#AM9342 |
| Denhard’s solution | Fisher | Cat#AAJ63135AD |
| tRNA from baker’s yeast | Roche | Cat#10109495001 |
| SDS 10% | Corning | Cat# 46-040-CI |
| UltraPure™ Salmon Sperm DNA Solution | Fisher | Cat# 15632011 |
| 4% PFA | EMS | Cat#157-4 |
| Rnase AWAY | Thermo Fisher Scientific | Cat#7002PK |
| Ethanol (200 Proof) | Fisher | Cat#BP2818500 |
| Critical commercial assays | ||
| Superscript IV | Thermo Fisher Scientific | Cat#18091050 |
| Effectene Transfection Reagents | QIAGEN | Cat#301425 |
| Pierce Coomassie Plus Assay Reagents | Thermo Fisher Scientific | Cat#1856210 |
| Deposited data | ||
| Experimental models: Cell lines | ||
| D. melanogaster: Cell line S2: S2-DRSC | Thermo Fisher Scientific | Cat#R69007 |
| Experimental models: Fly lines | ||
| D. melanogaster: w; Pdf-Gal4 | Park et al. 53 | N/A |
| D. melanogaster: w; UAS-mCD8::GFP | Bloomington Drosophila Stock Center | #32184 |
| D. melanogaster: yw; ; Pdf01 | Bloomington Drosophila Stock Center | #26654 |
| D. melanogaster: yw; Pdf-RescueB ; Pdf01 | Renn et al. 46 | N/A |
| D. melanogaster: yw | Bloomington Drosophila Stock Center | #1495 |
| D. melanogaster: w; ; eya-Gal4 | Bloomington Drosophila Stock Center | #49292 |
| D. melanogaster: w; UAS-Pdfr-RNAi | Bloomington Drosophila Stock Center | #38347 |
| D. melanogaster: w; UAS-t-PDF | Bloomington Drosophila Stock Center | #81111 |
| D. melanogaster: w; UAS-t-SCR | Bloomington Drosophila Stock Center | #81113 |
| D. melanogaster: w; UAS-eya-RNAi | Vienna Drosophila Resource Center | #108071 |
| D. melanogaster: w; per-AID-GFP | Chen et al.61 | #108071 |
| D. melanogaster: w; UAS-eya-RNAi | Vienna Drosophila Resource Center | #108071 |
| D. melanogaster: Pdfr-Gal4 , w | Bloomington Drosophila Stock Center | #33070 |
| D. melanogaster: w ; dilp2-Gal4 / Cyo | Bloomington Drosophila Stock Center | #37516 |
| Oligonucleotides | ||
| Primers for eya, see Methods details | This paper | N/A |
| Primers for cbp20, see Methods details | This paper | N/A |
| Library for Pdf detection, see Table S1 | Long et al.82 | N/A |
| Recombinant DNA | ||
| Plasmid: pMT-V5 | Thermo Fisher | Cat#V412020 |
| Plasmid: pMT-PKA-C1-V5 | This Paper | N/A |
| Plasmid: pAc-eya-3XFLAG-6XHis | Abrieux et al.24 | N/A |
| Software and algorithms | ||
| Fiji ImageJ (used for analysis of immunofluorescence microscopy images) | NIH Image | https://fiji.sc |
| GraphPad Prism 8 for Mac OS X | GraphPad Software | https://graphpad.com |
| Excel | Microsoft | Version 16.67 |
| R | R Core Team | Version 4.0.3 |
| RStudio | PBC | Version 2022.07.2 |
| CircaCompare | Parsons et al.55 | Release 0.1.1 |
| RAIN | Thaben & Westermark56 | Release 3.16 |
| Other | ||