| Antibodies |
| anti-FLAG-HRP (mouse monoclonal) |
Sigma |
A8592 |
| anti-FLAG (mouse monoclonal) |
Sigma |
F3165 |
| anti-HA |
Sigma |
3F10 |
| anti-ProtA |
Sigma |
P3775 |
| anti-FLAG magnetic beads |
Sigma |
M8823 |
| Protein G Dynabeads |
ThermoFisher |
10003D |
|
|
|
| Bacterial and virus strains |
| Rosetta 2(DE3)pLysS; E. coli
|
EMD Millipore |
71403 |
|
|
|
| Chemicals, peptides, and recombinant proteins |
| Lambda phosphatase |
Hinshaw stock |
N/A |
| IAA (3-Indoleacetic acid) |
Sigma |
I2886 |
| Benzonase |
EMD Millipore |
70746 |
| [γ-32P]ATP |
Perkin Elmer |
BLU502A |
| SYTOX Green Nucleic Acid Stain |
ThermoFisher |
S7020 |
| SYBR Gold Nucleic Acid Gel Stain |
ThermoFisher |
S11494 |
| SYBR Green 2× Master Mix |
ThermoFisher |
A46012 |
| 1-Napthaleneacetic acid |
Sigma |
N0640 |
| Phostag acrylamide |
APExBIO |
F4002 |
|
|
|
| Experimental models: Cell lines |
| High Five cells; Trichoplusia ni
|
ThermoFisher |
B85502 |
| Sf9 cells; Spodoptera frugiperda
|
ThermoFisher |
11496015 |
|
|
|
| Experimental models: Organisms/strains |
|
S. cerevisiae strains used in this study |
See Table S2
|
N/A |
|
|
|
| Oligonucleotides |
| ATCGAGAATCCCGGTGCC |
IDT |
oSMH1950 |
| ATCGGATGTATATATCTGACACGTGC |
IDT |
oSMH1951 |
| ATCAGCGCCAAACAATATGGAAAA |
IDT |
CEN3_fwd |
| GAGCAAAACTTCCACCAGTAAACG |
IDT |
CEN3_rev |
| AACTTCCAAAATGAAGGTCA |
IDT |
CUP1_fwd |
| GCATGACTTCTTGGTTTCTT |
IDT |
CUP1_rev |
|
|
|
| Recombinant DNA |
| Plasmids used in this study |
See Table S1
|
N/A |
|
|
|
| Software and algorithms |
| COMET |
Seattle Proteome Center |
https://uwpr.github.io/Comet/
|
| Graphpad Prism v9 (graphpad.com) |
Dotmatics |
N/A |
