Figure 10.

The trafficking of SP-pH-bearing DCVs is unaltered in Syt9 KO striatal neurons. A, Top, Representative image of a 90 µm region of a striatal neuron transfected with SP-pH; image was captured after perfusion with NH₄Cl. Bottom panels, Color inverted kymographs of this region, over a 30 s time period; each track in the kymograph was traced and quantified in the bottom panel (yellow lines). B, Quantification of the displacement and direction of SP-pH vesicles. No significant differences were detected between WT (n = 98 vesicles, from 5 cells) versus Syt9 KO (n = 101 vesicles, from 5 cells) striatal neurons (two-way ANOVA; F_(4,40) = 1.903, p = 0.1289).