Figure 8.

Unchanged synaptic density in Syt9 KO striatal neurons. A, Representative images of cultured striatal neurons stained with α-Gephyrin (red) and α-VGAT (green) antibodies with a neurite mask generated from MAP2 staining (magenta). Gephyrin and VMAT overlap within the neurite mask were counted (purple puncta within synapse mask). Scale bars, 20 μm. B, Number of synapses per micrometer is unaltered in cultured Syt9 striatal neurons (n = 15; 0.823 ± 0.0476 synapses/μm) compared with WT neurons (n = 15; 0.837 ± 0.0434 synapses/μm) (p = 0.8212; unpaired t test t = 0.228, df = 28).