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. 2023 Mar 7;6:245. doi: 10.1038/s42003-023-04615-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a 24 h after γ-irradiation (20 Gy) HEK-293T cells were treated with 2.5 µM cycloheximide for the indicated time points (0–8 h) and then CD47 surface expression was determined by Flow cytometric analysis. b CD47 cell-surface and intracellular staining (MFI) in HEK-293T cells 24 h after γ-irradiation. Graphs show an average (±STD) of four replicates in each group. c, d qRT-PCR analysis for CD47 expression 24 h after γ-irradiation in HEK-293T cells (c) and CHO_CD47 cells (d) following γ-irradiation. Graphs show an average (± STD) of three replicates in each group. One of at least three independent experiments is shown. **p < 0.005.