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. 2023 Mar 7;6:245. doi: 10.1038/s42003-023-04615-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative histograms of γH2AX staining before and after γ-irradiation. b Time-dependent expression of γH2AX (MFI) following γ-irradiation (20 Gy). c Representative dot plot of γH2AX and CD47 co-staining before and after γ-irradiation. d Cells were treated as above and after 24 h lysed. Western blot analyses were performed on whole cell extracts using antibodies to CD47 and γH2AX. Anti-β-actin immunoblotting revealed relative amounts of protein in each lane. Graph on the right shows an average (±STD) level of CD47 expression in triplicate samples normalized to actin in each sample (right panels). e The level of CD47 expression in response to γ-irradiation correlates with the level of residual γH2AX (left panel), and representative pictures taken by ImageStream of cells co-stained for CD47 and γH2AX before (0) and after γ-irradiation (5 and 20 Gy). f Graph depicts CD47 expression and γH2AX level (MFI) in HEK-293T cells 1,4 and 9 days after γ-irradiation. g The level of CD47 expression in response to different genotoxic agents correlates with the level of residual γH2AX. Graph shows an average (±STD) of four replicates in each group. One of at least three independent experiments is shown.