Figure 7.

SPP1⁺ profibrotic macrophages expand in human CKD and heart failure

(A) UMAP embedding of 4,404 mononuclear phagocytes sub-clustered from CD10⁻ single cells from 15 human kidneys by Kuppe et al.⁶ Labels refer to clusters. cDC, conventional dendritic cells; Mono, monocytes; Res-like Mac, resident-like macrophages; SPP1 Mac, SPP1⁺ macrophages.

(B) Bar plot of cluster cell numbers in CKD versus healthy kidneys after normalization via Log2 transformation. Log2FC, log 2-Fold Change.

(C) RNA-ISH for SPP1 and COL1A1 combined with immunofluorescent CD68 staining in human kidney nephrectomies. SPP1⁺CD68⁺ macrophages are circled in white. Scale bar = 30 μm.

(D) Pearson correlation of the number of COL1A1⁺ fibroblasts with SPP1⁺CD68⁺ macrophages in human kidney nephrectomies (n = 41).

(E) UMAP embedding of 20,892 mononuclear phagocytes sub-clustered from CD45⁺ single cells from six human heart samples from Rao et al.⁵⁰ Labels refer to clusters. Inflam. Mac, inflammatory macrophages.

(F) Bar plot of cluster cell numbers in heart failure versus healthy hearts after normalization via Log2 transformation. Log2FC, log 2-Fold Change.

(G) Cardiac ECM regulator score stratified by immune cell type.

For (B) and (F), Fisher’s exact test was computed using false discovery rate correction for multiple testing. For (G), a two-tailed unpaired t test was performed. ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001. See also Figure S7.