REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mouse CD11b (APC)	eBioscience	Cat# 17-0112-83; RRID: AB_469344
Anti-mouse mCD25 (PE-Cy7)	Biolegend	Cat# 102016; RRID: AB_312865
Anti-mouse CD4 (PB)	Biolegend	Cat# 100428; RRID: AB_493647
Anti-human CD68	Agilent	Cat# M0876; RRID: AB_2074844
Anti-mouse-Fc (AF488)	Jackson ImmunoResearch	Cat# 715-545-151; RRID: AB_2341099
mUromoduline	abcam	Cat# ab207170; RRID: AB_2889163
mPDGFRα	R&D Systems	Cat# AF1062; RRID: AB_2236897
LTL-Fitc	Vector Labs	Cat# FL1321; RRID: AB_2336559
mKIM1	R&D Systems	Cat#: AF1817; RRID: AB_2116446
mCD68	abcam	Cat# ab53444; RRID: AB_869007
Anti-rabbit-Fc (AF647)	Dianova	Cat# 111-605-008; RRID: AB_2338074
Anti-goat-Fc (Cy3)	Dianova	Cat# 705-165-147; RRID: AB_2307351
Anti-goat-Fc (AF647)	Dianova	Cat# 705-605-147; RRID: AB_2340437
Anti-rat-Fc (AF647)	Dianova	Cat# 712-605-153; RRID: AB_2340694
Bacterial and virus strains
pBABE-puro SV40 LT vector	Addgene	#13970
Biological samples
Human nephrectomy tissue samples (healthy and CKD)	This paper	N/A
Chemicals, peptides, and recombinant proteins
LPS	Sigma-Aldrich	L4391-1MG
Thrombine	Molecular-Innovations	MTHROM-0.05MG
Critical commercial assays
RNA-Scope™ Multiplex Fluorescent V2 Assay	ACD	323100
10x genomics single nuclear RNA-seq kit	10x genomics	1000077
Pikro Siriusred staining kit	Morphisto	13422
Cell Tracker CMFDA Dye	Thermo Fisher	C2925
Cell Tracker CMTPX Dye	Thermo Fisher	C34552
CD11b-Microbeads	Miltenyi Biotech	130-049-601
CD117-Microbeads	Miltenyi Biotech	130-091-224
High-Capacity cDNA Reverse Transcription Kit	Thermo Fisher	43-688-13
iTaq Univer SYBR Green Supermix	Biorad	1725125
Deposited data
Data scRNAseq MI hearts (murine)	Forte et al.23	E-MTAB-7895
Data of human healthy and CKD kidney	Kuppe et al.6	10.5281/zenodo.4059315
Data of human heart samples	Rao et al.50	GSE145154
Reference mapping onto Dataset of Sanin et al.	Sanin et al.38	GSE171328, GSE157313
Reference mapping of snRNA-Seq Data of IRI kidney onto murine IRI-snRNA-Seq dataset	Kirita et al.41	GSE139107
Raw-Data snRNA-Seq IRI kidneys (WT + Cxcl4−/−)	This paper	ENA: PRJEB58150
Bulk-Sequencing Data of Monocyte-Platelet-Coculture	This paper	ENA: PRJEB58150
Original Code	This paper	https://github.com/KramannLab/Spp1MacFibrosis/
Experimental models: Cell lines
Murine Cardiac Gli1+ cells	This paper	N/A
Experimental models: Organisms/strains
Cxcl4一/一 (Black-Six-tm(Cxcl4)) mice	Gift from Wasmuth, Aachen	Zaldivar et al. 201056
Gli1CreERt2 mice	Jackson Laboratories (Bar Harbor, ME, USA)	JAX Stock #007913
Rosa26tdTomato mice	Jackson Laboratories (Bar Harbor, ME, USA)	JAX Stock # 007909
Oligonucleotides
RNA-Scope detection probe hCol1a1 (C1)	ACD	401891
RNA-Scope detection probe C2-hSPP1	ACD	420101-C2
RNA-Scope detection probe C2-mC1qc	ACD	496451-C2
RNA-Scope detection probe C3-mSPP1	ACD	435191-C3
For qPCR primers, see Table S6	N/A	N/A
Software and algorithms
ImageJ	Schneider et al.70	imagej.nih.gov/ij/download.html
Ilastik	Berg et al.71	ilastik.org/download.html
RStudio Desktop		rstudio.com/products/rstudio/download/
GraphPad Prism version 9.0	N/A	graphpad.com/scientific-software/prism/
Scripts for image processing and analysis	This paper	https://github.com/thePowder/Cxcl4_image_analysis.git
Other
8-well μ-Slides	Ibidi	80827