Fig. 6. SOX11 is a core regulatory circuitry transcription factor in adrenergic NB.

a Binding of SOX11 (CUT&RUN), HAND2, PHOX2B, GATA3 (GSE90683)⁴, MYCN, TWIST1 (GSE94822)⁴⁶ and ASCL1 (GSE159613)⁴⁷ and at the SOX11 locus and downstream enhancer landscape. Signal represents log likelihood ratio for the ChIP and CUT&RUN signal compared to input signal (RPM normalised). Super-enhancers of CLB-GA are annotated using ROSE (red bar) showing the SOX11 consensus super-enhancer (SE). b Binding of SOX11 to the PHOX2B, HAND2, GATA3, ASCL1, TCF3 and TWIST1 locus. Signal represents log likelihood ratio for the ChIP and CUT&RUN signal compared to input signal (RPM normalised). c Tscore representing differential expression of MES and ADRN core regulatory circuitry transcription factors (TFs) in RNA-sequencing data after SOX11 knockdown (KD) in IMR-32, CLB-GA and NGP. Significant adjusted p-values are indicated with coloured bars. Statistical testing was done using the empirical Bayes quasi-likelihood F-test. d Heatmap profiles −2 kb and +2 kb around the summit of SOX11 CUT&RUN peaks in IMR-32, grouped for promoters or enhancers (homer annotation). On these regions HAND2, PHOX2B, GATA3 (GSE90683)⁴, MYCN, TWIST1 (GSE94822)⁴⁶ and ASCL1 (GSE159613)⁴⁷ ChIP data is mapped and ranked according to the sums of the peak scores across all datasets in the heatmap. e ISL1, GATA3, PHOX2B, TBX2, HAND2 and SOX11 (log2) expression during induced differentiation of hPSC cells along the sympatho-adrenal lineage. Expression levels depicted starting from day 16, upon sorting the cells for SOX10 expression indicating cells committed to truncal neural crest cells, and followed-up during sympatho-adrenal development until day 25. For Fig. 6c and e source data are provided as Source Data file.