FIG. 2.

Specific Notch proteins activate a subset of HMG domain transcription factors. (A) Ability of Notch 1, 2, and 3 to potentiate LEF-1. LEF-1 activity was assayed in NIH 3T3 cells using the LEF-OT reporter in the presence of Notch1 (N1) NICD, full-length Notch1 (FL N1), Notch2 (N2) NICD, or Notch3 (N3) NICD. Values are given as fold induction relative to LEF-OT alone. (B) Effects of NICD on other HMG box transcription factors. Neuro-2A cells were transfected with LEF-OT and expression vectors for LEF-1, TCF-1, HAF-2, or HAF-1 in the presence (striped bars) or absence (black bars) of NICD. Values are give as fold induction as for panel A.