FIG. 4.

NICD activates LEF-1 independently of CSL. (A) Schematic diagrams of the NICD deletion fragments are shown. R, RAM domain; A, Ankyrin repeats; TAD, C-terminal transcription activation domain; VP16 A.D., VP16 transcription activation domain. (B) NICDΔTAD-VP16 does not augment activity of LEF-1. Neuro-2A cells were transfected with a CSL-dependent reporter, CSL-luc (black bars), or LEF-OT (gray bars) and the expression vectors as indicated. NICDΔTAD-VP16 carries the VP16 TAD in place of the Notch TAD. Results are given as fold induction relative to the reporters alone. (C) The Notch TAD is sufficient for LEF-1 activation. NIH 3T3 cells were transfected with LEF-OT (gray bars) or LEF-OF (black bars) and the NICD fragments indicated. Results are presented as fold induction relative to the reporter alone.