FIG. 3.

Inhibitors of the NHE (but not the anion exchangers) prevent intracellular alkalinization following IL-7 or IL-3 withdrawal. (A) D1 cells were cultured in the presence or absence of IL-7 for 5 h and either not treated (None) or treated with 200 μM DMA or EIPA (inhibitors of the NHE). (B) D1 cells were cultured in the presence or absence of IL-7 for 5 h and either not treated (None) or treated with 200 or 1,000 μM DIDS or 1,000 μM SITS (inhibitors of the anion exchangers). (C) FL5.12A cells, incubated with or without IL-3 for 3 h, were either not treated (None) or treated with 200 μM DMA or EIPA (inhibitors of the NHE). (D) FL5.12A cells, incubated with or without IL-3, were either not treated (None) or treated with 200 or 1,000 μM DIDS or 1000 μM SITS (inhibitors of the anion exchangers). The intracellular pH was assayed as described in Materials and Methods.