FIG. 10.

Deletion and mutation analysis of the hLXRα proximal promoter. (A) Luciferase reporters containing bp −2625 to +345, −2210 to +345, −1310 to + 345, or 560 to +345 of the hLXRα promoter were transfected into HepG2 cells in the presence or absence of CMX-hLXRα, CMX-RXRα, and 5 μM T1317. Luciferase activity was normalized for transfection efficiency using β-galactosidase activity. The data are expressed as fold activations in the presence of the indicated ligand versus in the absence of ligand and represent the average of triplicate experiments. (B) hLXRα promoter constructs were cotransfected into HepG2 cells along with CMX vector or CMX-VP16-LXRα in the presence of 5 μM T1317. Data are expressed as relative luciferase activities normalized to β-galactosidase activities and represent the average of triplicate experiments. (C) Mutations were introduced into individual LXREs in the bp −2625 to +345 hLXRα promoter construct by site-directed mutagenesis. Wild-type (WT) and mutant (Mut) reporters were transfected into HepG2 cells along with CMX-hLXRα and CMX-RXRα in the presence or absence of 1 μM GW3965. Data are expressed as luciferase activities normalized to β-galactosidase activities and represent the averages of triplicate experiments.