FIG. 8.

PPARγ and LXRα bind to response elements in the hLXRα promoter. Gel mobility shift assays were performed using in vitro-translated receptors and end-labeled oligonucleotide probes as described in Materials and Methods. (A) Direct binding of PPARγ/RXR heterodimers to a putative PPRE from the hLXRα promoter. (B) Direct binding of LXRα/RXR heterodimers to the LXRE-A, LXRE-B, and LXRE-C sites from the hLXRα promoter. (C) Competition for LXRα/RXR binding to LXRE-C. Unlabeled oligonucleotide was included in the binding reaction at the indicated molar excess.