Figure 11.

Higher LH/GnRH pulse frequency in Fmr1 KO females before and after ovariectomy. LH pulse frequency reflects GnRH neuron activity. (A) Frequent tail-tip whole blood sampling over 3 hours demonstrate higher pulse frequency of LH in Fmr1 KO female mice in diestrus. Representative profiles from WT control in the top and Fmr1 KO at the bottom (right side), pulse frequency calculated from pulse profiles using DynPeak (left); amplitude was determined by subtracting the LH value at the peak from the basal value prior to the onset of the pulse and averaged for each mouse (middle). (B) LH pulse in ovariectomized (OVX) animals. Statistical significance (p < 0.05), determined with t-test followed by Tukey’s post hoc test, is indicated with a *.