Figure 9.

GnRH neurons of Fmr1 KO females have more GABA_A receptors. (A) GnRH neurons (green) express Fmrp (red, top panels). (B) Representative GnRH neurons (green) from WT control (top panel) and Fmr1 KO mice (bottom panel) co-stained with GABAγ2 antibody (red). (C) Quantification of (B) Each point represents one animals and an average of 15-20 neuron per animal, and bars represent group means. Panels represent counts in the whole soma, along the first 15 μm segment of the process (1-15 μm) and second 15 μm segment (16-30 μm) from the soma, as indicated above. Statistical significance (p < 0.05), determined with t–test followed by Tukey’s post hoc test, is indicated with a *.