Fig. 4.

Deficit of H₂R causes decreased activity of glutaminergic neurons. (A) Spike waveforms and autocorrelation of putative pyramidal neurons. (B1) Mean firing frequency of glutamatergic neurons in the mPFC of Hrh2^fl/fl and CaMKIIα-Cre; Hrh2^fl/fl mice. (B2) Burst per minute (Left) and percentage of spikes in bursts (Right) in the mPFC of Hrh2^fl/fl and CaMKIIα-Cre; Hrh2^fl/fl mice. n = 29 cells from three Hrh2^fl/fl mice, n = 23 cells from three CaMKIIα-Cre; Hrh2^fl/fl mice. (C–H) Patch-clamp recordings of glutamatergic neurons from CaMKIIα-Cre mice injected in the mPFC with AAV-FLEX-scramble shRNA-GFP (AAV-CON) or AAV-FLEX-shHrh2-GFP (AAV-shHrh2). (C) A schematic diagram demonstrating recordings of mPFC glutamatergic neurons. (D–F) Action electrical properties, namely (D) the injected current inducing the first action potential (AP), (E) AP firing threshold, and (F) AP amplitude. (G) Representative AP firing patterns. (H) Frequency of APs evoked by injected currents from −20 to 180 pA. n = 29 cells from five CaMKIIα-Cre+ AAV-CON mice, n = 32 cells from six CaMKIIα-Cre+AAV-shHrh2 mice. **P ≤ 0.01, ***P ≤ 0.001. See also Dataset S1 for further statistical information.