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. 2023 Feb 21;120(9):e2220120120. doi: 10.1073/pnas.2220120120

Fig. 5.

Fig. 5.

Requirement for type III IFN receptor is B cell intrinsic. (A) RT-qPCR of Ifnlr1 in sorted IgM−IgD− B cells from the PP (black) and thymus (blue) normalized to Gapdh. (B) Proportion of thymic B cells from mixed bone marrow chimeras with the following phenotypes: Ly6C+, CXCR3+, and IgM−IgD−. (C) Flow cytometry of thymic B cells from Ifnlr1fl/fl and Mb1Cre/+Ifnrl1fl/fl showing isotype class-switched (IgM−IgD−) (Left) and Ly6C expression (Right). Numbers adjacent to outlined areas identifying the proportion of cells within the gate. (D) Quantification of the proportion of CD38+GL7+, CXCR3+, Ly6C+, and isotype class-switched (IgM−IgD−) B cells from Ifnlr1fl/fl and Mb1Cre/+Ifnrl1fl/fl mice located in the PP, spleen, or thymus. Each symbol (A, B, and D) represents an individual mouse; small horizontal lines indicate the group mean. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ****P ≤ 0.0001 [(A) Two-tailed unpaired Student’s t test, (B) One-way ANOVA with Tukey’s multiple comparisons test, (D) Two-way ANOVA with Sidak’s multiple comparisons test].