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. 2023 Feb 22;120(9):e2216810120. doi: 10.1073/pnas.2216810120

Fig. 3.

Fig. 3.

Depletion of SLC25A47 elevated whole-body energy expenditure. (A) CaIR-analysis of covariance (ANCOVA) analysis of Slc25a47Alb-Cre mice and littermate controls at 23 °C after 1 wk on a high-fat diet and at 30 °C after 3 wk on a high-fat diet. n = 6 for Slc25a47Alb-Cre, n = 11 for controls. (B) Food intake of mice in (A). (C) Locomotor activity of mice in (A). (D) Serum FGF21 levels of mice at 12 wk of age on a regular chow diet and a high-fat diet for 6 w. Regular chow diet; n = 16 for Slc25a47Alb-Cre, n = 10 for controls. High-fat diet; n = 16 for Slc25a47Alb-Cre, n = 10 for controls. (E) Relative hepatic Fgf21 expression of mice in (D). (F) Relative hepatic Fgf21 expression of mice on a regular chow diet at indicated ages. n = 6 for Slc25a47Alb-Cre, n = 4 for controls (2 wk), n = 8 for Slc25a47Alb-Cre, n = 14 for controls (4 wk), n = 14 for Slc25a47Alb-Cre, n = 14 for controls (7 wk), n = 16 for Slc25a47Alb-Cre, n = 9 for controls (12 wk). (G) Correlation between serum FGF21 levels and serum AST levels of mice at 2 wk (Left) and 4 wk of age (Right) on a regular chow diet. n = 6 for Slc25a47Alb-Cre, n = 4 for controls (2 wk), n = 8 for Slc25a47Alb-Cre, n = 11 for controls (4 wk). ns, not significant, by simple linear regression. B–F, ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001 by unpaired Student’s t test.