Fig. 1.

The m⁶A epitranscriptome in the adult mouse brain. (A) Experimental scheme. (B) Upper: Pie chart showing the percentage of m⁶A methylated transcripts in the hippocampal CA1, CA3, and DG regions when calculated against the corresponding input. Lower: m⁶A peak location is shown for the annotated transcript regions. The percentages are calculated from the total number of m⁶A peaks. (C) Guitar plot showing the distribution of m⁶A peaks along mRNAs in the hippocampal subregions. (D) Venn diagram comparing m⁶A methylated transcripts across hippocampal subregions. (E) GO term (biological function) analysis of the m⁶A methylated transcripts commonly detected in all hippocampal regions.(G) Guitar plot showing the distribution of m⁶A peaks along mRNAs in the ACC. (F) Upper: Distribution of m⁶A RNA methylation across the transcriptome in the ACC. Lower: m⁶A peak location is shown for the annotated transcript regions. The percentages are calculated from the total number of m⁶A peaks. (H) Venn diagram comparing m⁶A commonly methylated transcripts in the hippocampus and ACC. (I) Sunburst plots showing the enrichment of synapse-specific GO terms within commonly m⁶A methylated transcripts in the hippocampus and ACC. (J) Pie chart showing the percentage of m⁶A methylated transcripts within RNA-seq datasets obtained from hippocampal synaptosomes or synapses isolated from microfluidic chambers. ACC - anterior cingulate cortex, DG - dentate gyrus, 5UTR - 5′ untranslated region, 3UTR - 3′ untranslated region, CDS - coding sequence, ncRNA - noncoding RNA.