Fig. 2.

Conserved m⁶A modifications between mouse and human. (A) Upper: Pie chart showing the percentage of m⁶A methylated transcripts in the human CC calculated against the corresponding input. Lower: m⁶A peak location is shown for the annotated transcript regions. The percentages are calculated from the total number of m⁶A peaks. (B) Guitar plot showing the distribution of m⁶A modifications along mRNAs in the human CC. (C) Motif analysis within the m⁶A peaks identifies the m⁶A DRACH consensus motif (D = A, T, or G, R = A or G, and H = A, T, or C). (D) Left: Doughnut chart showing the mouse/human genes with known homologues in human/mouse, respectively, that were used to compare methylated transcripts across species. Right: Venn diagram comparing m⁶A methylated homologue transcripts in the adult mouse ACC to the human CC. (E) GO categories (biological process) for m⁶A methylated transcripts common to the mouse ACC and human CC. (F) Sunburst plot showing synapse-specific location GO term enrichment for m⁶A methylated transcripts common to the mouse ACC and human CC. (G) Representative coverage tracks showing conserved m⁶A modifications along the 3′ end of homologous transcripts, in this case Camk2b in the mouse ACC and human CC (CAMKIIb/CAMKIIB). Tracks show coverage values for m⁶A-RIP normalized for the corresponding inputs and library size. Scale in RPM. (H) GO categories (biological process) detected when m⁶A methylated transcripts specific to the human CC are analyzed. (I) Heat map showing the odds ratio for the association between conserved transcripts (commonly detected in mice and humans) and human- and mouse-specific transcripts in comparison to synaptic RNAs, as published in ref. 34. Color scale represents the numerical value of enrichment (odds ratio), numbers in orange correspond to the P value for the corresponding overlap, and numbers in parentheses refer to the number of overlapping genes. N.S. = not significant. SC = RNAs detected in the synaptic compartments of microfluidic chambers; Syn = RNAs detected in synaptosomes (34). Random corresponds to 2,000 randomly selected brain-expressed human genes. ACC - anterior cingulate cortex, CC - cingulate cortex.