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. 2023 Feb 24;120(9):e2211823120. doi: 10.1073/pnas.2211823120

Fig. 4.

Fig. 4.

Two example case with no clear structural similarity (A) and a case with low sequence identity (B). Human chromosome 11 open reading frame 53 (C11orf53) was an uncharacterized protein in March 2022. A recent paper (39) experimentally showed that it is a transcriptional coactivator of POU2F3 and plays a role in the generation of Tuft cell lineage. The putative homolog found by PROST is POU2AF1, which is a transcriptional coactivator that associates POU2F1 and POU2F2. C11orf53 has a similar function with the POU2AF1 found by PROST, validating the homology relationship. PROST identifies a putative homolog for human MKRN2 opposite strand protein (H3BPM6) with a 16.4% sequence identity. (A) Structures predicted by AlphaFold2 (29) for C11orf53 (Left) and POU2AF1 (Right) show no clear similarity. (B) Structures predicted by AlphaFold2 (29) for H3BPM6 (Left) and Q8BVA2 (Right). The alignment of these structures has a FATCAT p-value of 2.91e-03 and a 0.47 TM-Score. (C) Sequence alignments of C11orf53 and its putative homolog POU2AF1 have 22.2% sequence identity. (D) Sequence alignments of human MKRN2 opposite strand protein (H3BPM6) and Q8BVA2 have 16.4% sequence identity.