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. Author manuscript; available in PMC: 2023 Mar 8.
Published in final edited form as: Curr Opin Chem Biol. 2022 Nov 30;72:102232. doi: 10.1016/j.cbpa.2022.102232

Figure 2. Pn DAH7PS-CM enzyme compaction in response to substrate and product binding.

Figure 2.

Top panels compare Pn DAH7PS-CM SAXS analysis for ligand-free state (black) with addition of DAH7PS substrates/cofactor, PEP/Mn2+ (red) or PEP/Mn2+/E4P (blue), via A) Kratky and B) Porod-Debye plots. The bottom panels demonstrate lack of change when CM product prephenate is bound alone (black), in the presence of PEP/Mn2+ (red), or PEP/Mn2+/E4P (blue), via C) Kratky and D) Porod-Debye plots. Panels A–D: adapted under permission (copyright Elsevier, 2021).[15] E) AlphaFold2 models of Pn DAH7PS-CM illustrating compaction expected upon DAH7PS substrate binding (not fit to original data).