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Dissociated MAS98.12/MAS98.12PR PDX tissue; appearance and proliferative capacity ex vivo. Tumors were disintegrated using collagenase/DNAse with/without gentleMACS. The resulting tissue suspension was stained with trypan blue (A) to identify dead cells and EdU (B) to evaluate the proliferating capacity. The EdU staining was also performed on 7/11 day-cultures either in suspension (B) or in Matrigel (C); DAPI stains the nucleus; scale bars, 100 μm.
