Figure 5.

Sensitivity of MAS98.12- and MAS98.12PR-derived PDXCs to paclitaxel. Untreated and paclitaxel treated for one week PDXCs in Matrigel were stained with calcein/PI and a proportion of viable cells was quantified. (A) Representative pictures, where the red lines in the phase contrast pictures (left) mark the automatically detected fragment area, and the green line marks the “live” part, as validated by the fluorescence pictures (middle); scale bar, 200 μm. (B) A proportion of viable cells in the treated cultures presented as a percentage of the respective untreated controls; average ± SEM (n=4; where either fresh (n=2) or cryopreserved (n=2) PDX tissue was used to establish PDXCs, see Supplementary Figure S1 ); *, p < 0.05 by unpaired t-test.