Reagent	Final concentration	Amount (for 50 mL)
DMEM/F-12	1 part	48.4 mL
N2 supplement	1×	0.5 mL
Insulin-transferrin-selenium	1×	0.5 mL
Penicillin/streptomycin	1×	0.1 mL
L-glutamine	2 mM	0.5 mL