Fig. 6.

Displacement of Fis from fimS by the Lrp protein. Either 0 or 270 nM of purified Fis protein was prebound to a 135 bp fragment of fimS DNA containing the LRP-1, LRP-2 binding sites and the Fis binding site. Increasing concentrations of purified Lrp protein were added to the Fis–fimS complex and resolved by electrophoresis. Control lanes containing fimS DNA with no protein, with just Fis, or with just Lrp were included as controls. Arrowheads indicate the unbound fimS probe, the Fis–fimS complex and the Lrp–fimS complexes. At 220 nM, Lrp almost completely displaced prebound Fis from fimS.