Table 1.
Bacterial strains and plasmids
|
Strain/plasmid |
Relevant details |
Reference/source |
|---|---|---|
|
Strains |
||
|
VL386 |
φ(fimA-lacZ)λpL(209)fimE::IS1 |
[9] |
|
VL386recD |
VL386 recD::Tn10 |
[24] |
|
CJD2116 |
VL386 Δfis::kan |
This work |
|
CJD2117 |
VL386 Δlrp::cml |
This work |
|
CJD2119 |
CJD2116 (pFIS349) |
This work |
|
VL386fimS-dist |
VL386 with Fis/LRP-2 binding site disrupted |
This work |
|
XL-1 Blue |
recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F' proAB lacIqZ M15 Tn10 (Tetr)] |
Stratagene |
|
Plasmids |
||
|
pSGS501 |
fimB::kan, fimE::IS1, φ(fimA-lacZ) cloned in pACYC184, switch phase OFF Cmr |
[24] |
|
pFIS349 |
pGS349 containing the Salmonella enterica serovar Typhimurium dusB-fis operon, Apr |
[77] |
|
pLSB124 |
pMAK705 containing wild-type fimB and sacB gene from Bacillus subtilis |
This work |
|
pMAK705 |
Temperature-sensitive allele exchange vector |
[109] |
|
pMCL210 |
Cloning vector, p15A replicon |
[110] |
|
pMMC106 |
pMCL210 cut with NheI and XbaI and religated to delete the lac promoter |
[20] |
|
pMMC108 |
fimS cloned as a 550 bp fragment in the PstI site of pMMC106 |
[20] |
|
pSLD203 |
fimB gene cloned in pUC18 |
[28] |
|
pUC18 |
ColE1 replicon, Apr |
[111] |