Table 2.
Name |
Sequence |
Purpose |
---|---|---|
OL4 |
5′-GACAGAACAACGATTGCCAG-3′ |
PCR switch assay |
OL20 |
5′-CCGTAACGCAGACTCATCCTC-3′ |
PCR switch assay |
BSFORBIO |
5′-CCACCTCATGCAATATAAAC-3′ |
Probe for gel retardation assays |
BSREVBIO |
5′-CCCCCAAAAGATGAAACATTT-3′ |
Probe for gel retardation assays |
SpvR11 |
5′-CCAAGCTTCAGTACTGATCTTGCGATACTG-3′ |
Probe for gel retardation assays |
SpvR14 |
5′-CCCAAGCTTCAGGTCACCGCCATCCTGTTTTTGC-3′ |
Probe for gel retardation assays |
COL-DFP |
5′-GAGAAGAGGTTTGATTTAAC-3′ |
Probe for DNase I footprinting |
COL69 |
5′-GAGTTTGACTGCCAACACT-3′ |
Probe for DNase I footprinting and primer for Sanger sequencing |
FBSFOR2 |
5′-CAATAGAATATTAAGGGGTTAGCTAAACT- GAAAAAG-3′ |
Mutagenesis of Fis binding site |
FBSREV2 |
5′-CTTTTTCAGTTTAGCTAACCCCTTAATATTCTATTG-3′ |
Mutagenesis of Fis binding site |