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. 2023 Jan 12;169(1):001283. doi: 10.1099/mic.0.001283

Table 2.

Oligonucleotides

Name

Sequence

Purpose

OL4

5′-GACAGAACAACGATTGCCAG-3′

PCR switch assay

OL20

5′-CCGTAACGCAGACTCATCCTC-3′

PCR switch assay

BSFORBIO

5′-CCACCTCATGCAATATAAAC-3′

Probe for gel retardation assays

BSREVBIO

5′-CCCCCAAAAGATGAAACATTT-3′

Probe for gel retardation assays

SpvR11

5′-CCAAGCTTCAGTACTGATCTTGCGATACTG-3′

Probe for gel retardation assays

SpvR14

5′-CCCAAGCTTCAGGTCACCGCCATCCTGTTTTTGC-3′

Probe for gel retardation assays

COL-DFP

5′-GAGAAGAGGTTTGATTTAAC-3′

Probe for DNase I footprinting

COL69

5′-GAGTTTGACTGCCAACACT-3′

Probe for DNase I footprinting and primer for Sanger sequencing

FBSFOR2

5′-CAATAGAATATTAAGGGGTTAGCTAAACT-

GAAAAAG-3′

Mutagenesis of Fis binding site

FBSREV2

5′-CTTTTTCAGTTTAGCTAACCCCTTAATATTCTATTG-3′

Mutagenesis of Fis binding site