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The HDAC inhibitor TSA accelerates cyclin A transcription. NIH 3T3 cells were transiently transfected with cyclin A promoter-luciferase constructs (+CRE; 1 μg), pRL-SV40 Renilla luciferase (0.1 μg), and either E7 (1 μg) or empty vector (1 μg). Serum-starved transfectants (G0) were stimulated with 10% FCS in DMEM in the absence or presence of 50 nM TSA for 3 h. Luciferase activity was normalized to a constant activity of Renilla luciferase.
