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. 2023 Mar 8;20:62. doi: 10.1186/s12974-023-02729-6

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Chronic THC administration increased CRYM protein expression in the basal ganglia of chronically SIV-infected RMs. Basal ganglia tissues of uninfected control (A), VEH/SIV (B–D), and THC/SIV RMs (E–G) were immunostained for CRYM (green), NeuN (red), and DAPI for nuclear staining (blue). Note the significantly increased CRYM staining in NeuN⁺ neurons in the BG of THC/SIV (E–G) compared to VEH/SIV (B–D) and uninfected control RMs (A). A few NeuN⁻ cells expressing CRYM protein were also detected (E and G, white arrowhead). Representative immunofluorescence images were captured using a Zeiss confocal microscope at 20X magnification. Yellow staining (A, E) indicates colocalization of CRYM to NeuN⁺ neurons (white arrow). Quantitation of CRYM (H) signal intensity was performed using Halo software. Differences in CRYM signal intensity between groups were analyzed using unpaired “t” tests after confirming data assumptions (normal distribution) employing the Prism v9 software (GraphPad software). A p-value of < 0.05 was considered significant