KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-beta-tubulin, clone E7 | DSHB | catalog #E7; RRID: AB_528499 |
| Anti-Alpha-Tubulin-K40ac, Clone 6-11B-1 | Sigma-Aldrich | catalog #T7451; RRID: AB_609894 |
| anti-giantin | BioLegend | catalog #924302; RRID: AB_291560 |
| anti-FLAG tag agarose beads, clone M2 | Sigma-Aldrich | cat# A2220 |
| anti-FLAG tag | ThermoFisher | cat# MA1-91878-BTIN; RRID: AB_2537620 |
| Biological samples | ||
| porcine brain tubulin | Cytoskeleton | cat# T240 |
| bovine brain tubulin | R. Ohi lab | lab purified |
| biotinylated tubulin | Cytoskeleton | cat# T333P |
| fluorescent tubulins (HiLyte488, X-Rhodamine, HiLyte647) | Cytoskeleton | cat# TL488M, TL620M, TL670M |
| 3xFLAG peptide | Sigma-Aldrich | cat# F4799 |
| Chemicals, peptides, and recombinant proteins | ||
| Taxol | Cytoskeleton | cat# TXD01 |
| Neutravidin | ThermoFisher | cat# 3100 |
| GMP-CPP | Jena Sciences | cat# JBS-NU-405S |
| biotin-BSA | Sigma-Aldrich | cat# A8549 |
| Janelia Fluor 552 (JF552) | Janelia Materials | cat# JF552 |
| trichostatin A | Sigma-Aldrich | cat# T1952 |
| Experimental models: Cell lines | ||
| COS-7 | ATCC | RRID: CVCL_0224 |
| hTERT-RPE1 | ATCC | RRID: CVCL_4388 |
| MRC-5 | ATCC | RRID: CVCL_0440 |
| NIH-3T3 | ATCC | RRID: CVCL_0594 |
| U-2OS | ATCC | RRID: CVCL_0042 |
| Recombinant DNA | ||
| plasmid pN1-RnKIF5C(1-560)-3xmCit | Cai et al.62 | lab plasmid |
| plasmid pN1-RnKIF5C(1-560)-Flag | Budaitis et al.20 | lab plasmid |
| plasmid pN1-RnKIF5C(1-560)-Halo-Flag | this study | lab plasmid |
| plasmid pN1-RnKIF5C(1-560)-mNeonGreen | Norris et al.63 | lab plasmid |
| plasmid pN1-RnKIF5C(1-560,Δ6)-3xmCit | this study | lab plasmid |
| plasmid pN1-RnKIF5C(1–560,Δ6)-Flag | this study | lab plasmid |
| plasmid pN1-RnKIF1A(1-560,Δ6)-Halo-Flag | this study | lab plasmid |
| plasmid pN1-RnKIF5C(1-560,Δ6)-mNeonGreen | this study | lab plasmid |
| plasmid pN1-RnKIF1A(1-393)-3xmCit | Hammond et al.64 | lab plasmid |
| plasmid pN1-RnKIF1A(1-393)-Flag | Budaitis et al.19 | lab plasmid |
| plasmid pN1-RnKIF1A(1-393)-Halo-Flag | Budaitis et al.19 | lab plasmid |
| plasmid pN1-RnKIF1A(1-393)-mNeonGreen | Budaitis et al.19 | lab plasmid |
| plasmid pC1-mCitrine-MmalphaTAT | Soppina et al.65 | lab plasmid |
| plasmid pN1-RnKIF5C(1-560)-mNG-FRB | Schimert et al.66 | lab plasmid |
| plasmid pN1-GMAP210-mRFP-FKBP | Engelke et al.67 | lab plasmid |
| adenovirus pShuttle-EGFP-tubulin | Kumar et al.68 | Addgene#24327 |
| Software and algorithms | ||
| Fiji/ImageJ | Schneider et al.69 | https://fiji.sc/ |
| Prism 8.0 | GraphPad | https://www.graphpad.com/ |
| Other | ||
| carboxy polystyrene beads | Spherotech | cat# CP-10-10 |
| copper grids | EMS | cat# FCF400-CU |