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. Author manuscript; available in PMC: 2023 Mar 8.
Published in final edited form as: Cell Rep. 2022 Dec 30;42(1):111915. doi: 10.1016/j.celrep.2022.111915

Figure 4. Spontaneous somatodendritic dopamine release is not detectably impaired by Syt-1 cKODA.

Figure 4.

(A) Strategy for AAV-mediated overexpression of D2 receptors (human, short version [hD2RS]) in the midbrain.

(B–D) Example traces (B) and quantification of frequency (C) and amplitude (D) of spontaneous D2-IPSCs recorded in brain slices after viral expression of D2 receptors in basal recording conditions, after reducing extracellular Ca2+ from 2.4 to 0.5 mM, or following blockade of sodium channels with 1 μM TTX. Basal: Syt-1 control 17 cells/7 mice, and Syt-1 cKODA 18 cells/5 mice; 0.5 mM Ca2+: Syt-1 control 9 cells/4 mice, and Syt-1 cKODA 10 cells/5 mice; 1 μM TTX: Syt-1 control 7 cells/6 mice, and Syt-1 cKODA 7 cells/4 mice.

Data are mean ± SEM; no significant differences were detected with two-way ANOVA. For evoked D2-IPSCs after D2 receptor overexpression, see Figure S3.