KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| STING (D2P2F) | Cell Signaling | Cat# 13647, RRID:AB_2732796 |
| TBK1 / NAK | Cell Signaling | Cat# 3504, RRID:AB_2255663 |
| IRF3 | Cell Signaling | Cat# 4302, RRID:AB_1904036 |
| Phospho-STING (Ser366) | Cell Signaling | Cat# 19781, RRID:AB_2737062 |
| Phospho-TBK1 (Ser172) (D52C2) | Cell Signaling | Cat# 5483, RRID:AB_10693472 |
| Phospho-IRF-3 (Ser396) (D6O1M) | Cell Signaling | Cat# 29047, RRID:AB_2773013 |
| TP53 | Santa Cruz Biotechnology | Cat# sc-126, RRID:AB_628082 |
| p53 (CM5) | Leica | Cat# sc-6243, RRID:AB_653753 |
| GAPDH | GeneTex | Cat# GTX100118, RRID:AB_1080976 |
| Anti-beta-Actin-Peroxidase antibody | Sigma-Aldrich | Cat# A3854, RRID:AB_262011 |
| c-Myc | Santa Cruz Biotechnology | Cat# sc-40, RRID:AB_627268 |
| HA-probe (F-7) | Santa Cruz Biotechnology | Cat# sc-7392, RRID:AB_627809 |
| GFP (B-2) | Santa Cruz Biotechnology | Cat# sc-9996, RRID:AB_627695 |
| Flag-HRP | Sigma-Aldrich | Cat # A8592, RRID:AB_439702 |
| anti-mouse CD335 (NKp46) antibody | BioLegend | Cat# 137611, RRID:AB_10915472 |
| anti-mouse CD45 | Biolegend | Cat# 103131, RRID:AB_893344 |
| CD3e Monoclonal Antibody (145-2C11) | Thermo Fisher Scientific | Cat# 25-0031-81, RRID:AB_469571 |
| CD4 Monoclonal Antibody (RM4-5) | Thermo Fisher Scientific | Cat# 17-0042-81, RRID:AB_469322 |
| anti-mouse CD8a | BioLegend | Cat# 100707, RRID:AB _312746 |
| TREX1 | Abcam | Cat# ab185228, RRID:AB_2885196 |
| TRIM24 | Proteintech | Cat# 14208-1-AP, RRID:AB_2256646 |
| V5-probe (E10) | Santa Cruz Biotechnology | Cat# sc-81594, RRID:AB_1131162 |
| cGAS | Cell Signaling | Cat# 15102, RRID:AB_2732795 |
| Anti-Lamine B1 | Abcam | Cat# ab16048, RRID:AB_443298 |
| dsDNA | Santa Cruz Biotechnology | Cat# sc-58749, RRID:AB_783088 |
| IFI-16 (1G7) | Santa Cruz Biotechnology | Cat# sc-8023, RRID:AB_627775 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Lipofectamine 2000 | Invitrogen | Cat# 2082816 |
| Lipofectamine RNA iMAX | Invitrogen | Cat# 13778-150 |
| PicoGreen | Invitrogen | Cat# P7589 |
| Cyclosporine A | ApexBio | Cat# B1922 |
| Ethidium bromide | Sigma-Aldrich | Cat# 15585-04 |
| HT-DNA | Sigma-Aldrich | Cat# D6898 |
| Doxycycline hyclate | Sigma-Aldrich | Cat# D9891 |
| Sodium pyruvate | Gibco | Cat# 11360-070 |
| Hoechst 33342 | ThermoFisher Scientific | Cat# H3570 |
| DAPI | ThermoFisher Scientific | Cat# 62248 |
| FITC Annexin V/ PI | BioLegend | Cat# 640914 |
| Doxycycline hydrochloride | Fisher Scientific | Cat# BP2653-5 |
| Halt™ Protease Inhibitor Cocktail | ThermoFisher Scientific | Cat# 78429 |
| Fluoromount G | SuthernBiotech | Cat# 0100-01 |
| Pro Long™ Gold Antifade | ThermoFisher Scientific | Cat# P10144 |
| BSA | GoldBiotechnology | Cat# A-420 |
| Doxorubicin hydrochloride | Sigma | Cat# D1515 |
| Critical Commercial Assays | ||
| BCA Protein Assay Kit | Pierce | Cat# 23235 |
| Mouse IFN Beta ELISA Kit | pbl | Cat# 42400-1 |
| Human IFN Beta ELISA Kit | pbl | Cat# 41410-1 |
| SsoAdvanced Universal SYBR® Green | Bio-Rad | Cat #1725274 |
| QIAprep spin Miniprep Kit | Qiagen | Cat# 27106 |
| Reasy Mini Kit | Qiagen | Cat# 74104 |
| CellTiter-Blue® Cell Viability Assay | Promega | Cat# G8081 |
| AnnexinV-FITC/PI apoptosis detection kit | BiLegend | Cat# 640914 |
| Clarity Max Western ECL Substrate | Bio-Rad | Cat# 1705061 |
| Exonuclease activity assay kit | VioVision | Cat# K175-100 |
| NE-PER™ Nuclear and Cytoplasmic Extraction Reagents | ThermoFisher Scientific | Cat# 78835 |
| cGAMP ELISA Kit | Cayman chemical | Cat# 501700 |
| ChIP-IT Express Enzymatic | Active Motif | Cat# 53009 |
| Senescence β-Galactosidase Staining Kit | Cell Signaling | Cat# 9860 |
| Experimental Models: Cell Lines | ||
| A549 | ATCC | N/A |
| 4T1 | ATCC | N/A |
| CT26 | ATCC | N/A |
| H1299 | ATCC | N/A |
| Experimental Models: Organisms/Strains | ||
| BALB/c | Envigo | Cat # 4702F |
| NOD/SCID | Envigo | Cat # 1700M |
| Deposited data | ||
| Uncropped Western Blot Data | Mendeley | DOI: 10.17632/y84khfx263.1 |
| Software and Algorithms | ||
| GraphPad Prism 8.0 | GraphPad Software, Inc. | https://graphpad.com/scientific-software/prism/ |
| Excel 2016 | Microsoft | https://www.office.com/ |
| ImageJ 1.52a | Wayne Rasband, NIH | https://imagej.net/ |
| BD FACS DIVA 6.2 | BD Biosciences | https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-cell-sorters/facsaria-iii |
| Kaluza | Beckman Coulter Life Sciences | https://www.beckman.com/flow-cytometry/software/kaluza |
| NIS-elements AR 5.02.01 | Nikon | https://www.microscope.healthcare.nikon.com/ |
| Leica TCS SP8 X confocal | Leica | https://www.leica-microsvstems.com |