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. Author manuscript; available in PMC: 2023 Oct 28.
Published in final edited form as: Science. 2022 Oct 28;378(6618):eabm3233. doi: 10.1126/science.abm3233

Figure 3. Isolation and identification of a previously undescribed genotoxic metabolite derived from M. morganii.

Figure 3.

(A) Overview of isolation and identification of genotoxins derived from M. morganii.

(B) Proposed 4 candidate ion features initially detected from M. morganii cultures. Rt, retention time.

(C) Evaluation of nicking of circular pUC19 DNA (top band = nicked DNA) after co-incubation overnight with F1–F4 fractions enriched with ion features I–IV, respectively. Ctrl, control pUC19 DNA in TE buffer.

(D) Chemical structures of compounds indolimine-214 (1) and 2.

(E) MFI of γ-H2AX staining for HeLa cells treated with synthetic compounds at indicated concentrations for 5 h. n.s., not significant; * p<0.05; ** p<0.01; *** p<0.001, two-way ANOVA.

(F) Genomic DNA comets in HeLa cells after treatment with 100 μg/ml synthetic compounds for 5-6 h. n = 25 for comet assay analysis, n.s., not significant; * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001, one-way ANOVA.