Fig. 4. diaPASEF quantitative proteomics for molecular glue 9. MM.1S cells were treated with compound 9 at 0.1 μM for 3 h. Bioconductor's limma package was used to perform statistical analysis of degrader treatment compared to DMSO vehicle treatment. The identified proteins were plotted as log 2 fold change (PROTAC/DMSO) versus −log 10 of the p-value. Proteins with −log 10 (p-value) > 3 (p-value < 0.001) and log 2 fold change > 0.6 or < −0.6 (translating to 1.5-fold up- or down-regulation) were considered to have significantly changed in abundance. Data are the mean of biological duplicates.