REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
E. coli BL21-DE3 RIL	Agilent	230245
E. coli MG1655	Coli Genetic Stock Center	CGSC6300
Phage T2	German Collection of Microorganisms and Cell Cultures GmbH (DSMZ)	DSM 16352,Accession: LC348380.1
Phage T4	U. Qimron	Accession: AF158101.6
Phage T6	German Collection of Microorganisms and Cell Cultures GmbH (DSMZ)	DSM 4622,Accession: MH550421.1
Phage T5	U. Qimron	Accession: AY543070.1
Phage T7	U. Qimron	Accession: NC_001604.1
Lambda vir	U. Qimron	Accession: NC_001416.1
Chemicals, peptides, and recombinant proteins
Ni-NTA Agarose	Qiagen	30250
HiTrap Q HP Column	Cytvia	17115401
Zorbax Bonus-RP	Agilent	863668-901
SRT SEC-300	Sepax	215300-7830
Nuclease P1 from Penicillium citrinum	Sigma-Aldrich	N8630
Alkaline Phosphatase, Quick CIP	New England Biolabs	M0525S
Quantifoil R 2/1 300 mesh grids, copper	Electron Microscopy Sciences	FCF400-Cu
Recombinant DNA
pBbE8k-RFP	Lee et al.39	Addgene Cat#35276
pSG1	Doron et al.5	N/A
pBAD-GFP	Thermo Fisher Scientific	Cat #43001
E. coli P0304799.3 RADAR	Genscript Corp.	N/A
C. rodentium DBS100 RADAR	Genscript Corp.	N/A
S. suis SS993 RADAR	Genscript Corp.	N/A
EcRADAR_A_K82A	Genscript Corp.	N/A
EcRADAR_A_E151K	Genscript Corp.	N/A
EcRADAR_A_N155R	Genscript Corp.	N/A
EcRADAR_A_K148E	Genscript Corp.	N/A
EcRADAR_A_K150E	Genscript Corp.	N/A
EcRADAR_A_R162E	Genscript Corp.	N/A
EcRADAR_B_E501A	Genscript Corp.	N/A
EcRADAR_B_K85E	Genscript Corp.	N/A
EcRADAR_B_E595/596/597R	Genscript Corp.	N/A
EcRADAR_B_D141R	Genscript Corp.	N/A
EcRADAR_B_K393E	Genscript Corp.	N/A
EcRADAR_B_R250E	Genscript Corp.	N/A
EcRADAR_B_F282R	Genscript Corp.	N/A
EcRADAR_B_D21R	Genscript Corp.	N/A
EcRADAR_B_T396R	Genscript Corp.	N/A
Deposited data
EcRdrA unsplit	This paper	EMD: 29323, PDB: 8FNT
EcRdrA single split	This paper	EMD: 29324
EcRdrA double split	This paper	EMD: 29325
SsRdrA	This paper	EMD: 29326, PDB: 8FNU
EcRdrB	This paper	EMD: 29327, PDB: 8FNV
EcRdrA–EcRdrB	This paper	EMD: 29328, PDB: 8FNW
Oligonucleotides
Primers, see Table S5	This paper	N/A
Software and algorithms
Phenix 1.13-2998	Adams et al.40	https://www.phenix-online.org/
Coot 0.8.9	Emsley and Cowtan41	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Pymol v1.7.4.4	Schrödinger, LLC	https://pymol.org/
Prism 7.0d	GraphPad software	https://www.graphpad.com/scientific-software/prism/