FIG. 3.

(A) HBx-induced DNA binding activities of several transcription factors. EMSA was carried out in the presence of γ-³²P-labeled oligonucleotides representing cognate sequences for the transcription factors NF-κB, NF-AT, AP-1, CRE, and NF-1 cognate sequences and the nuclear lysates prepared from untransfected and cells transfected with wild-type HBx expression vector. Lanes 1 and 2, equal amounts of untransfected and HBx-transfected nuclear lysates; lane 3, a 100-fold excess of respective unlabeled oligonucleotides as competitors. (B) EMSA with NF-1 probe with untransfected HepG2 lysates. Lane 1, probe alone; lane 2, equal amount of untransfected nuclear lysates; lanes 3 to 6, HBx-transfected nuclear lysates; lane 4, cotransfected with Mn-SOD expression vector; lanes 5 and 6, treated with PDTC and NAC.