FIG. 2.

Rvb2p is required for accumulation of box C/D and H/ACA snoRNAs in yeast. Test cells dependent on a GAL::rvb2 allele (strain YWD313) were grown in galactose medium and shifted to glucose medium. Total RNA was extracted from cells harvested at various intervals following the shift, and snoRNAs were examined by Northern blot analysis. RNA loading was adjusted to obtain approximately equal amounts of tRNA per lane. The gel patterns show the effects of Rvb2p depletion on the accumulation of box C/D snoRNAs (A), box H/ACA snoRNAs (B), and RNase P, MRP, and 5S RNAs (C).